FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641927
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8250.041.5773351
GTATCAA20500.038.9863551
TCGTATG400.002562651634.58963840
CTCGTAT501.844211E-434.55163639
TATCAAC24050.033.2316172
ATCAACG24150.033.0940063
TCAACGC24250.032.816094
CAACGCA24650.032.146935
AACGCAG25000.031.696876
TGGTATC3250.029.551892
ACGCAGA26900.029.4557637
AGAGTAC27450.029.11549411
CAGAGTA28000.028.66608810
CGCAGAG27950.028.5946468
GCAGAGT28850.027.8215059
ATACGAT500.0079021727.47743430
GTGGTAT3450.025.8502561
CGAGACG1351.0006261E-825.40837323
AGTACGG22950.025.40639113
GAGTACG23450.024.8646812
Produced by FastQC (version 0.11.5)