FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001237
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT704.4929038E-1044.30135337
ACGAGAC3400.043.1703922
GCGTATG758.458301E-1041.5301340
TCGTATG1350.041.0174140
GTATCAA41700.040.9207531
CCCACGA3850.039.00332319
TAAGGCG3900.038.5958129
GGTATCA19100.038.2375531
GACTAAG3900.037.66959826
CGTATGC1950.037.29707341
TATCAAC45900.037.102022
TATGCCG1950.035.65293543
CGAGACT4250.035.34654623
TCAACGC48600.035.04084
ATCAACG48850.034.8614653
AATCGCG1201.8189894E-1234.42709736
CAACGCA50500.033.8592835
AACGCAG50950.033.3592726
ATCTCCG4600.032.64245610
TCTCCGA4850.032.36712311
Produced by FastQC (version 0.11.5)