FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1853
Sequences flagged as poor quality0
Sequence length25-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAACTCTTCCAGTTTTGCCATGGTAACACTTCTGGGACATTCCTTT30.16189962223421478No Hit
GTAAATGCGTTAACTAGGCTTTAAATGACGCAATTCTCCCTGCGTCATGG30.16189962223421478No Hit
CCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCCAA30.16189962223421478No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.16189962223421478No Hit
CTCCTGTAGGGCCTAGATATTTATCAGCATGGCCAAGGCCTGGGGG20.10793308148947653No Hit
TCATTGAAGTCCTTGGAATTATTTTTCAAATTCTGTTTCTTTGAATCTTT20.10793308148947653No Hit
GTCAGAAGAAAACCAACTGGACTCTAAATTACACACACCTTAATGACAAG20.10793308148947653No Hit
AACTGACAGTCACCTCCCAGAACCTGCAGCTGGAGAACCTGCGCATGAAG20.10793308148947653No Hit
GTTATGGCAAAATCAATAAGAAGCGAATTGCTTTGACAGATAACGCTTTG20.10793308148947653No Hit
TGGATGCACCGAGATCGGCAGTGCCAGGTACGTGATCCACGGCAGCACGG20.10793308148947653No Hit
CACGAGAACATGCCTCTCGCAAAGGATCTCCTTCATCCCTCTCCAGAAGAGGAGAAGAGGAAACACAAGAAGAAA20.10793308148947653No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20.10793308148947653No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGG20.10793308148947653No Hit
GCGGAAGGCTGAGAGGAAGAGAGGGCGTGAGGGGCGGACGACTACTTTTG20.10793308148947653No Hit
GCGACTACCTGTTCGCCACGCGCGGCGGCAAGAAGATCCCGCTGTACGGTGGCTGCGGCGGACCGGGCTACTTCA20.10793308148947653No Hit
AAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGT20.10793308148947653No Hit
GAGTACGGGAAGAAGAGTGGGACACCCCCAGGGGGAGTCCAAGGAGGCCTGGCCTGGAGAAGAGTGAGGTTACC20.10793308148947653No Hit
CGCGGGGGCCGAGTCGCGAAGCGCGCCTGCGACCCGGCGTCCGGGCGCGC20.10793308148947653No Hit
AGCTGGGGGTCAGCAGGCTGGGGGCGGCTGTCCAGACTCCCCTGGCCCCATGCCCTCAGGGAGGGAGCGGCAGAT20.10793308148947653No Hit
GACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCC20.10793308148947653No Hit
GCTTCAGCATCGGCTTGGCGATCACGATGGTTGCCGCGGGCGTCGTCGCT20.10793308148947653No Hit
CAGGCTTCGAGCTGAACGTCTGCGGAGGGAAGCAGCTGAGAGGTCTCGGG20.10793308148947653No Hit
GTCATAGCCAAGCAAAAGGTATCATCAAAGCTGCTCAACTCATATGGCCGAAAGAACTCATTGTGGATATCAAT20.10793308148947653No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCA20.10793308148947653No Hit
TAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCAC20.10793308148947653No Hit
GATGGTACATTCAGGCACCAGCATATTCCTATGCATTTCTAGATAAAAGT20.10793308148947653No Hit
AACAGTAAGTCAGACAACATTTGCCACAATAATGTCTGTCAAAGTGACTTGCCATAAACACCCCAGCACCACATT20.10793308148947653No Hit
CATCATCTCCTCGAACACTGCGACGGGGCGCACGCCCGGTGCCGACTTGA20.10793308148947653No Hit
GAGTACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGG20.10793308148947653No Hit
GAACAGAAGATGTTTCAGGTCTGTCACTGGAAGAACAATTGAGGAGGTTG20.10793308148947653No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTG20.10793308148947653No Hit
CAGGTTTTCTCTGTGTGTGAGGCAGAAGAGGCAACTTGTTTCAGCCTGTGAACTTCAAAGAAGAAAAGCAACCG20.10793308148947653No Hit
ACCATGTCCAGGAGAGCTTGCAGGATGAGGGCGCAGAGCCCACGGGCTAC20.10793308148947653No Hit
CTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAAC20.10793308148947653No Hit
GTATCAACGCAGAGTACGGGACCTAACAAACCCACAGGTCCTAAACTACC20.10793308148947653No Hit
TTTCCTTCCTCATGCTACTCTTCTAAGTCTTCATATTTTCTCTTAAAATC20.10793308148947653No Hit
TGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGG20.10793308148947653No Hit
TTCTTCCTTGGAGGAGGTCAGGATCTTCCCAAGGCCTGGGTCCTCGAATATTCTTCCAGTCATCAAACTTGGAGT20.10793308148947653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG20.10793308148947653No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAAAA150.001966345669.297360
Produced by FastQC (version 0.11.5)