FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1853
Sequences flagged as poor quality0
Sequence length23-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100.5396654074473827No Hit
CCAGTTTGCTGGCAAAGTAGAAAAGGGCGACAACCCGGCCCCAGTTGAAGTTGCCGTCAGAAAACATGTCAGCTG20.10793308148947653No Hit
CTATTTTTCTTTAAGAGAGAAATGTACGAACAGTACCCTTGATTATACCC20.10793308148947653No Hit
GTCTTGGGAAACACAAAAACAGTTTTTTAAAGCCAGTTACTAGATATCAT20.10793308148947653No Hit
GTACTAGTCAATAAACTTATATATTTTCTCCCCATTGCAGCACAACTAAG20.10793308148947653No Hit
CCCTATGATGAAGTTATCTGTGCTGCGAACTTAAAGAAGTTGATAGTGAA20.10793308148947653No Hit
GTTCTTTTGCCGCCAGCCCAGAGTCAGGCTACCTTAAGCGCCTGTCCTGTCTGAGACTGTGGCTGACATGTCTCC20.10793308148947653No Hit
GACTATGAGGAGGGCCCTGGGAAGAATTTGCCATTTTCAGTGGAAAACAA20.10793308148947653No Hit
TTGCTGGAGCCGGGCTCCCTCTACATCCTTAGGGGCTCAGCCCGTTATGA20.10793308148947653No Hit
CCTTCAGGGGGTCAGCATTCTGGAGCAGGTGCATCACATGGTCCTCTGTCATGTTGCCATACTTGGTGGCATT20.10793308148947653No Hit
CCGTAAAGGCGCTGCGCGCCTGCGCGCCGTGGATTTCGCTGAGCGGCACGGCTACATCAAGGGCATCGTCAAGGA20.10793308148947653No Hit
GGTATCAACGCAGAGTACGGGCTCTCGGCCTTAGCGCCATTTTTTTGGAA20.10793308148947653No Hit
GCGTTGGCGGGCGTGGCGGTGCCGGCCACCAGGTCGACCGGGCCGAAGGC20.10793308148947653No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10793308148947653No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10793308148947653Clontech SMART CDS Primer II A (100% over 21bp)
GTGCTTGAGAGTGAGCCTTTCGAAGAGATACTCGCCCAGCCCAGCCTCCGGGCCACCCAGCCTGTGGAGGTTGGT20.10793308148947653No Hit
CATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGG20.10793308148947653No Hit
ACGTGAGGTGCCAAGAAAAATGCCTGACACATTGAAAGTGCTGGATAAAT20.10793308148947653No Hit
ATACATCACTGTGATAGAATGTGGGCTTTTTCAAGGGTGAAGATACAAGT20.10793308148947653No Hit
GTGCTGCCAAGGGCATCAAGACGATGCTGGTATCACTTCCCTATAGTGAGTCGTATTA20.10793308148947653Clontech Universal Primer Mix Short (95% over 21bp)
CTCAAGGGTCGTGTGTTTGAAGTGAGTCTTGCTGATTTGCAGAATGATGA20.10793308148947653No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10793308148947653Clontech SMART CDS Primer II A (100% over 21bp)
TTTTTCTTCATTCCACCTCGTGGAGAAGACAATTTGAAGGGCCACAGGAAGTTATTTGCCTCTTTGAAGCGTTTT20.10793308148947653No Hit
CAATGACAGGAGGGGGCAAAGGCCAAAGGATTCTGAAATTAGAAGAATAC20.10793308148947653No Hit
GTCTTCTGCACCCAACAAAAATCAGTTAGATGGTAAGAAAAAGAAACCAA20.10793308148947653No Hit
GTGCAACAGTGTTCTCATCTTTCCAGGCAGGCAGATTATTTTAATGCTGT20.10793308148947653No Hit
GAGCTGGAAATGCAGCTATTGAGATCTTCGAATGCTGCGGAGCTGGAGGC20.10793308148947653No Hit
CCCCCAGGCCTTGGCCATGCTGATAAATATCTAGGCCCTACAGGAG20.10793308148947653No Hit
CAATATATGTTCTCTAGGCCTTGGAAAAAACATGGAGTTGTTCCTTTGGC20.10793308148947653No Hit
ATTATAAGGTGGATGAGAATGGCAAAATTAGTCGCCTTCGTCGAGAGTGC20.10793308148947653No Hit
GTGCTTTTCGCAAGAACAAGACTCTCGGCTATGGAGTCCCCATGTTGTTGCTGATTGTTGGAGGTTCTTTTGGT20.10793308148947653No Hit
CGGTGGTCGCGGTGGATCGGGTATGGAGCAAGGATCTTGTGAACGAGAGC20.10793308148947653No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT20.10793308148947653No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT20.10793308148947653No Hit
GAGTTACTGTAAAAGACGTGAACCAGCAGGAGTTCGTCAGAGCTCTGGCAGCCTTCCTCAAAAAGTCCGGGAAG20.10793308148947653No Hit
GTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAAGAGATTAA20.10793308148947653No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)