FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525691
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61450.039.8337021
GGTATCA26800.039.4720951
GTGGTAT8150.035.2848431
TATCAAC69400.035.2212942
ATCAACG72450.033.7407573
TCAACGC74450.032.927414
CAACGCA75050.032.6641655
AACGCAG75450.032.5374376
ACGCAGA82100.029.8960677
CGCAGAG82650.029.697128
AGAGTAC82050.029.57760211
CAGAGTA84050.028.95619610
TGGTATC9900.028.7018182
GCAGAGT87750.028.0101479
AGTACGG55900.026.57413113
GAGTACG56000.026.52754412
ACGGGTT4050.026.20004516
TCTATAC1707.2759576E-1226.1811943
TACGGGT11950.024.91910615
TCGTATG2950.024.6190240
Produced by FastQC (version 0.11.5)