FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525691
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31380.20567729638570326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8750.041.075221
GTATCAA63750.040.108741
GGTATCA27650.037.3861241
TATCAAC72050.035.313332
ATCAACG74650.034.0711023
TCAACGC76300.033.328854
CAACGCA76700.033.1550335
AACGCAG77750.032.839176
ACGCAGA83200.030.6880447
TGGTATC11400.030.598142
AGAGTAC84250.030.18387411
CGCAGAG85000.030.0783928
CAGAGTA85200.029.7670810
GCAGAGT88600.028.93349
GAGTACG60900.026.26639412
AGTACGG61100.026.01259213
TACGGGT11350.023.79047615
TAACGGC2200.023.48386236
AGCGTAA750.001913387823.16241543
GAGTACT40750.023.06625212
Produced by FastQC (version 0.11.5)