FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1305158
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35100.037.0300031
GGTATCA18150.034.7666471
TATCAAC42650.030.7160932
ATCAACG43350.030.299213
TCAACGC43600.030.1266334
CAACGCA44200.029.7952635
AACGCAG44100.029.7862016
ACGCAGA47800.027.4805767
ACGTACG500.00795542927.44172920
GTACTAA1005.287875E-727.4354211
AGAGTAC47750.026.86188711
CGCAGAG49650.026.3875488
CAGAGTA48950.026.20437610
GCAGAGT52900.024.8312199
CTCGTAT1653.0322553E-923.07233439
TAGCGAA902.2144672E-422.86285215
AGTACGG40000.022.29127913
GAGTACG40050.022.1786712
CGATTTA800.002626596321.93303157
GTGGTAT7450.021.1749881
Produced by FastQC (version 0.11.5)