FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605401
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTCTTGTTGGTACTGTATAAAACATT14370.2373633343849779No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14150.2337293793700374No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGCAATCCGGGGTCCCT13210.21820248066983702No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12070.19937198650150892No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11450.18913084055031293No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10440.17244768343626785No Hit
GTACCAACAAGAGGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGG9500.15692078473606752No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9090.15014841402640564No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8570.14155906580927352No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8470.13990726807520965No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8350.13792511079433303No Hit
GTTTTATACAGTACCAACAAGAGGAACTACTTAGCTTGGTACCAGCAGAA7950.13131791985807753No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7930.13098756031126477No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7870.12999648167082645No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGGCACTGAATCGGTC7430.12272857164094542No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7420.12256339186753903No Hit
GCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC7170.11843389753237936No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7040.11628656047809632No Hit
CCTCTGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG6790.11215706614293666No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6580.10868829090140254No Hit
GTCCCTGACCGATTCAGTGCCAGCGGGTCTGGGACAGATTTCACTCTCAC6400.10571505498008758No Hit
TTATAGTACTCCTCTGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT6400.10571505498008758No Hit
GTCCCAGACCCGCTGGCACTGAATCGGTCAGGGACCCCGGATTGCCGGGT6210.10257663928536624No Hit
ATATTATAGTACTCCTCTGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT6110.10092484155130237No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6080.1004293022310832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)