FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101088
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14950.13577479729140632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG31550.033.109793
TCAACGC31950.032.6967544
CAACGCA32500.032.143435
AACGCAG32950.031.7044456
GTATCAA34500.031.6745171
TCGTATG802.69245E-630.28570640
AGAGTAC36300.028.4906711
TATCAAC38200.028.4253582
ACGCAGA36750.028.4235787
CAGAGTA37650.027.65286610
CGCAGAG38250.027.308938
GCAGAGT38950.027.0827889
GTGGTAT4800.026.488811
TATACAC2350.026.3200593
GGTATCA17850.025.9894751
AGTACGG27950.024.46324313
GAGTACG28050.024.3760312
TTATACG750.002032323722.92277527
TAACACG750.002039314722.909244
CGAGACT2351.8189894E-1221.93637323
Produced by FastQC (version 0.11.5)