FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856010
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55100.040.2889751
GGTATCA24500.039.7113881
TATCAAC62600.035.2987982
ATCAACG67300.032.9372333
TCAACGC67550.032.9176564
CAACGCA68250.032.530725
AACGCAG69650.032.1236656
CGTATGC4200.030.55596241
ACGCTAA1901.7515049E-830.45220870
TCGTATG4150.030.05388640
GTGGTAT8500.029.8246651
ACGCAGA77200.028.8457
TATACGG605.646987E-428.5522335
CGCAGAG78300.028.4397748
AGAGTAC77700.028.34768711
CTCGTAT3950.028.02748939
CAGAGTA80200.027.63562810
TCTCGTA4250.026.82734338
GCAGAGT84000.026.5092149
CTTATAC5600.025.0817971
Produced by FastQC (version 0.11.5)