FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994548
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51160.5144045335167332No Hit
AGCTTACACTGGTACCAGCAGAAACCAGGTCAGTCTCCAAAGCTCCTCAT35150.353426883368123No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29900.30063908428753566No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28310.28465192228027203No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27210.27359162152052996No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25580.2572022667583666No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25250.253884176530444No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC24810.24946005622654713No Hit
GAGTAGTAGTTTTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT24440.2457397732437248No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22640.22764109927323767No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22420.22542903912128928No Hit
GTCCTTATCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATATGGGACAGATTTCACCCTCACCATCAATAGC21890.22009998511886808No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21690.2180890213443695No Hit
GATTTCACCCTCACCATCAATAGCCTGGAAGCTGAAGATGCTGCAATGTATTTCTGTCATCAGAGTAGTAGTTTT21080.21195558183214885No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20230.20340898579052996No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19410.19516403431508586No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19020.19124265495481366No Hit
TCCCAGTCCTTATCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATATGG18370.1847070226876933No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA17520.1761604266460744No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16760.16851876430297985No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16620.16711108966083085No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16450.16540177045250706No Hit
GTGTAAGCTACTACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGACTTTCTCCTTTGGAGTCACAGACTGGAA16220.16308916211183372No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16160.16248587297948414No Hit
CTACTACTCTGATGACAGAAATACATTGCAGCATCTTCAGCTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTC16020.16107819833733514No Hit
GTTCCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACCTGCCGGGCCAGTCAGAGCATTGGTAGTAGCTTACA15590.15675462622216324No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15590.15675462622216324No Hit
CTCCAAAGCTCCTCATCAAGTTTGCTTCCCAGTCCTTATCAGGGGTCCCC15570.15655352984471338No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14780.14861022293544404No Hit
GTCTCCAGAGTTCCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACCT14590.14669980734967042No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14100.1417729461021489No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13840.13915869319530078No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC13720.13795211493060164No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13560.13634334391100278No Hit
GTATTTCTGTCATCAGAGTAGTAGTTTTCCTCAGACGTTCGGCCAAGGGA13360.13433238013650423No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13350.1342318319477793No Hit
GGATATGGGACAGATTTCACCCTCACCATCAATAGCCTGGAAGCTGAAGA12990.13061209715368186No Hit
CACCATCAATAGCCTGGAAGCTGAAGATGCTGCAATGTATTTCTGTCATCAGAGTAGTAGTTTTCCTCAGACGTT12920.12990825983260737No Hit
GTTCAGTGGCAGTGGATATGGGACAGATTTCACCCTCACCATCAATAGCC12880.12950606707770768No Hit
GGGTTCCAGCCTCCAGGGGTGAAATTGTGCTGACTCAGTCTCCAGAGTTC12860.12930497070025782No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12820.1289027779453581No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12790.1286011333791833No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12620.12689181417085954No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12330.1239759166978366No Hit
GATAAGGACTGGGAAGCAAACTTGATGAGGAGCTTTGGAGACTGACCTGGTTTCTGCTGGTACCAGTGTAAGCTA11860.11925015182776498No Hit
ATATGGGACAGATTTCACCCTCACCATCAATAGCCTGGAAGCTGAAGATG11620.11683699529836669No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11240.11301616412681942No Hit
GAGCAAAACTGACAAGTCAAGGCAGGAAGATGTTGCCATCACAACTCATTGGGTTTCTGCTGCTCTGGGTTCCAG11240.11301616412681942No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11040.11100520035232085No Hit
GGACTGGGAAGCAAACTTGATGAGGAGCTTTGGAGACTGACCTGGTTTCT10840.10899423657782228No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10810.10869259201164751No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10660.10718436918077358No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10580.10637998367097415No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10520.10577669453862458No Hit
GCTCTGGGTTCCAGCCTCCAGGGGTGAAATTGTGCTGACTCAGTCTCCAG10380.10436901989647558No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10100.10155367061217759No Hit
CTGCTGCTCTGGGTTCCAGCCTCCAGGGGTGAAATTGTGCTGACTCAGTC10060.10115147785727788No Hit
AAACTGACAAGTCAAGGCAGGAAGATGTTGCCATCACAACTCATTGGGTT10030.1008498332911031No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)