FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1957339
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT79680.4070832901199026No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG56330.28778867636112093No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG54050.2761402087221478No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52340.26740385799291794No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCCGCCGAAAGTG51090.26101763669962125No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC47210.24119480580522845No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47210.24119480580522845No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG46860.23940666384310536No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45700.23348025048292603No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42490.21708043420174022No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT39510.20185568263852097No Hit
GACCAGGGAAAGCCCCTAAGCTCCTGATCTACCATACATCCAATTTGGAA38940.1989435657287777No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTGGGAGACAGAGTCACCATAACTTGCCAGGCGAGTCAGGACGTTAGCGA38340.1958781795079953No Hit
GATTTTACTCTCACCATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAATCGTCAGATAATTTC37470.1914333694878608No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG37460.19138227971751443No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT36950.1887767014298494No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC36540.18668202084564808No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33460.1709463715789651No Hit
ATATTACTGTCAATCGTCAGATAATTTCCCCCTCACTTTCGGCGGGGGGA33280.1700267557127304No Hit
GAGTAAAATCCGTCCGAGATCCACTTCCACTGAACCTTGATGGGACCCCT32950.16834079329130008No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTAAAATCCGTCCGAGATCCACTTCCA32940.16828970352095368No Hit
GTCTGCGTCTGTGGGAGACAGAGTCACCATAACTTGCCAGGCGAGTCAGG32720.1671657285733335No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG32420.1656330354629423No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31730.16210784130904254No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC31080.15878700623652828No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT30660.1566412358819806No Hit
GTGGGAGACAGAGTCACCATAACTTGCCAGGCGAGTCAGGACGTTAGCGACTATTTAAATTGGTATCAGCAGAGA27940.14274481834776706No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTAAAATCCGTCCGAGATCCACTTCCA27530.14065013776356575No Hit
AGTCAGGACGTTAGCGACTATTTAAATTGGTATCAGCAGAGACCAGGGAA27220.13906635488282817No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27090.1384021878683253No Hit
ACATATTACTGTCAATCGTCAGATAATTTCCCCCTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAA26260.13416173692957634No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26120.1334464801447271No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25990.13278231313022423No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25560.1305854530053302No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24950.12746897701420143No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24250.12389269308995529No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24090.12307525676441332No Hit
GCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATCTACCATACATCCAATTTGGAAACAGGGGTCCCATCAAGGTT24080.12302416699406696No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24050.12287089768302784No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAATCGTCAGATAATTTCCCCCTCACTTT23770.12144038411332936No Hit
ACCATACATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTCGGACGGATTTTACTCTCA23560.12036749893605553No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23490.12000987054363091No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23450.11980551146224543No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT23380.1194478830698208No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22850.11674012524146302No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC22670.11582050937522831No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22420.11454326511656898No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCGGATGACCCAGTCT21930.11203986636959667No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21690.11081371188128374No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21540.11004736532608812No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG21120.10790159497154045No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCGGATGACCCAGTCTCCATCCTCC21020.1073906972680767No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCGGATGACCCAGTCTCCATC20660.10555146553560728No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20560.10504056783214354No Hit
GCCTGAAGATATTGCAACATATTACTGTCAATCGTCAGATAATTTCCCCC20270.1035589644920987No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19980.10207736115205389No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)