FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1957339
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT80340.4104552149627632No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG53400.2728193736496335No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC52440.2679147556963817No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCCCGCCGAAAGTG52210.26673969097841504No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49910.2549890437987492No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT48130.24589506467709477No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45250.23118121081733925No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40810.2084973527835495No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40030.20451235069653237No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC39510.20185568263852097No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT38050.19439657616795045No Hit
GACCAGGGAAAGCCCCTAAGCTCCTGATCTACCATACATCCAATTTGGAA36740.18770381625257557No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTGGGAGACAGAGTCACCATAACTTGCCAGGCGAGTCAGGACGTTAGCGA36530.18663093107530174No Hit
GATTTTACTCTCACCATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAATCGTCAGATAATTTC36310.1855069561276815No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33940.17339868055559102No Hit
GTGGGAGACAGAGTCACCATAACTTGCCAGGCGAGTCAGGACGTTAGCGACTATTTAAATTGGTATCAGCAGAGA33880.1730921419335128No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33590.17161053859346798No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT33080.16900496030580292No Hit
GAGTAAAATCCGTCCGAGATCCACTTCCACTGAACCTTGATGGGACCCCT32820.16767662627679722No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG32300.1650199582187858No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31050.15863373692548913No Hit
ATATTACTGTCAATCGTCAGATAATTTCCCCCTCACTTTCGGCGGGGGGA30750.15710104381509796No Hit
GTCTGCGTCTGTGGGAGACAGAGTCACCATAACTTGCCAGGCGAGTCAGG30490.15577270978609223No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTAAAATCCGTCCGAGATCCACTTCCA29380.1501017452776448No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC28240.14427751145815823No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28060.14335789559192352No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27320.1395772525862919No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTAAAATCCGTCCGAGATCCACTTCCA27080.13835109809797894No Hit
AGTCAGGACGTTAGCGACTATTTAAATTGGTATCAGCAGAGACCAGGGAA26180.13375301876680534No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26120.1334464801447271No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25560.1305854530053302No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT25450.13002346553152008No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25340.12946147805771No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25130.12838859288043614No Hit
ACATATTACTGTCAATCGTCAGATAATTTCCCCCTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAACGAA24600.12568083505207836No Hit
ACCATACATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTCGGACGGATTTTACTCTCA24570.12552756574103924No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT23960.12241108974991045No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23890.12205346135748586No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23670.12092948640986564No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23610.1206229477877874No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23490.12000987054363091No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23260.11883480582566433No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23110.11806845927046875No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22720.11607595822696018No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAATCGTCAGATAATTTCCCCCTCACTTT22710.11602486845661379No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22520.1150541628200327No Hit
GCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATCTACCATACATCCAATTTGGAAACAGGGGTCCCATCAAGGTT22310.11398127764275887No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21990.11234640499167492No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCGGATGACCCAGTCT21720.11096698119232284No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCGGATGACCCAGTCTCCATCCTCC20930.10693088933495934No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20890.10672653025357386No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20610.1052960166838754No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20470.10458075989902618No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20350.10396768265486969No Hit
GCCTGAAGATATTGCAACATATTACTGTCAATCGTCAGATAATTTCCCCC19990.10212845092240025No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCGGATGACCCAGTCTCCATC19860.10146428390789741No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19640.1003403089602772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4350.042.6749761
GTATCAA36850.042.2599951
TATCAAC41800.037.2262
ATCAACG44550.034.6037643
TCAACGC44850.034.1365364
CAACGCA45150.033.9097185
AACGCAG46700.033.0047536
CGCAGAG50400.030.5817858
AGAGTAC50150.030.52888511
CAGAGTA51500.029.9952410
GCAGAGT52800.029.0616769
ATTGACA29950.028.3379770
TCGATTA500.007732621427.60262935
ACGCAGA60600.025.3777087
AGTACGG43600.024.72174513
GAGTACG44450.024.32622512
GTGTATA2001.8189894E-1224.0639441
GTATGTA10900.022.7077851
AGTACTT20200.022.09163713
GAGTACT20950.021.4646212
Produced by FastQC (version 0.11.5)