FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640674
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACACCCGAGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATCCA39560.24112041758448055No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29100.17736613123630898No Hit
GTCTATTACTGTGCGAGAGATCGGGGATTCCTTCTTTGGAGTGGACCCCT29060.17712232899405975No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24560.14969457674102227No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23860.1454280375016609No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22740.1386015747186827No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22660.13811397023418423No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG20870.12720381989353155No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG20770.12659431428790852No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20710.1262286109245347No Hit
CTCCTGTGGTGCCTCTGGATTCAGCTTTAGTGCCTATTGGATGAACTGGGTCCGCCAGGCTCCTGGGAAGGGG20640.12580195700059854No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20360.12409534130485397No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19900.12129161551898793No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19890.12123066495842562No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGATAC19120.11653747179512809No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG18920.11531846058388198No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18850.11489180665994585No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18390.1120880808740798No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18270.11135667414733214No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17560.10702918434740845No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG17540.10690728322628384No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT17360.10581017313616232No Hit
AGTCAGGACATTAACAAAAATTTAAATTGGTATCAGCAAAAACCAGGGAA17120.10434735968266699No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16980.10349405183479472No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16510.10062937548836637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27250.036.6648371
GTGGTAT5050.034.7934151
TATCAAC31250.031.972712
TCAACGC31900.031.540124
ATCAACG32100.031.2343643
CAACGCA32850.030.6285
TCTGGAG20950.030.34167970
AACGCAG33900.029.6793466
CGTCTAA500.007719010627.61233733
GCGTTGT14250.026.79978862
GTATAGG1057.7802906E-726.2493721
CGCAGAG38200.026.246688
ACGCAGA38900.025.9515157
AGAGTAC38650.025.84879311
ACCCTGG16950.025.75891970
TCGGGTC15650.025.5365320
CAGAGTA39150.025.43222210
CGTTGTC15050.025.170563
TTGGCGT15000.025.15993759
TGGCGTT15200.024.85270160
Produced by FastQC (version 0.11.5)