FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640674
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACACCCGAGTCCTCGGGTCTCAGGCTGTTCATTTGCAGATCCA32460.19784551958524363No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27440.16724833818296628No Hit
GTCTATTACTGTGCGAGAGATCGGGGATTCCTTCTTTGGAGTGGACCCCT26700.16273799670135566No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23760.14481853189603786No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23550.14353857012422944No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22660.13811397023418423No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22250.13561499725112972No Hit
CTCCTGTGGTGCCTCTGGATTCAGCTTTAGTGCCTATTGGATGAACTGGGTCCGCCAGGCTCCTGGGAAGGGG21510.13110465576951913No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20880.12726477045409387No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG20270.12354678625979323No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG19860.1210478132767387No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19830.12086496159505178No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG19630.11964595038380568No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18610.11342899320645052No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18560.11312424040363898No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18340.11178332807126826No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGATAC18230.11111287190508291No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17460.10641967874178539No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGTATCCTGACGCT17180.10471306304604083No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16840.10264074398692244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28100.039.4722671
GTGGTAT5100.038.4988021
TATCAAC32750.033.6379432
TCAACGC34300.032.1985934
ATCAACG34150.032.1414683
CAACGCA34700.031.6291265
AACGCAG35550.030.7760946
AGAGTAC39450.027.90716611
CGCAGAG39400.027.7687858
CAGAGTA40350.027.62576110
TAGACCG500.00784026427.5242825
ACGCAGA40400.027.081447
GCAGAGT42250.026.302799
TTAACGG1001.5586493E-524.16106635
TCTGGAG19350.023.60686170
CGTTATG750.001756490423.50572265
GCGTTGT11950.023.1765562
CGTTGTC12450.022.55818263
TCGGGTC12250.022.47633620
GAGTACT17200.022.40280312
Produced by FastQC (version 0.11.5)