FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295664
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39550.040.992141
GGTATCA16650.039.5250931
TATCAAC44700.036.652732
CTACGCT1650.033.27811428
CAACGCA49450.033.065235
ATCAACG49550.032.9959533
TCAACGC49550.032.8576054
AACGCAG50200.032.639516
GTGGTAT4650.030.9586661
ACGCAGA56200.029.151497
AGAGTAC55800.028.8690711
CGCAGAG57000.028.5619548
CAGAGTA56600.028.52158410
GCAGAGT59100.027.5470649
AGTACGG41300.025.22662513
GAGTACG41550.024.9923612
TCGTATG700.001309198924.7221340
CGAATTA750.002072063722.8469215
TGGTATC5950.022.4664042
AGACTAC2450.022.4022725
Produced by FastQC (version 0.11.5)