FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295664
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22770.1757400066683955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39650.040.1844861
GGTATCA17750.037.83611
TATCAAC45250.035.2680242
GTGGTAT5600.033.0410921
CAACGCA50300.032.0490575
TCAACGC50450.031.8846954
ATCAACG50250.031.8766083
TGGTATC5700.031.8430612
AACGCAG50850.031.8370296
AGAGTAC54250.029.46203211
CGCAGAG55700.029.3095238
ACGCAGA55950.028.9961667
CAGAGTA55800.028.76631510
GCAGAGT57850.028.042759
TAACGGA500.00801286127.40128124
AGTACGG41200.024.83720813
GAGTACG41750.024.59198612
TATAGGG3900.022.8308752
TACGGGG18200.022.37971915
GTACGGG44700.021.973714
Produced by FastQC (version 0.11.5)