FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120720
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36040.16994228375268777No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC23540.11100003772303746No Hit
GTATAATAGTTACCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT23170.10925534724055981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65450.042.455051
TATCAAC73400.037.8784142
GGTATCA25500.036.9952961
ATCAACG77150.035.6590733
TCAACGC77500.035.4496424
CAACGCA78950.034.841965
AACGCAG81950.033.650086
GTGGTAT9350.033.020591
ACGCAGA87850.031.3901427
CGCAGAG88800.031.2472118
AGAGTAC89700.030.78093511
CAGAGTA90750.030.38704310
GCAGAGT93750.029.5973579
TGGTATC11050.027.6117972
ACGGGTA3150.026.1030416
AGTACGG59700.026.0507813
GAGTACG61100.025.734212
TCAAACG8500.024.61081548
GAGTACT48500.024.15591812
AGTACTT48650.024.0814413
Produced by FastQC (version 0.11.5)