FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1761495
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTGCGCCGAAAATCAC87800.4984402453597654No Hit
GACCTAGGACGGTCAGCTTGGTCCCTGCGCCGAAAATCACGTTGTTGCGTGTATATAAACTGCAGTAATAATCAG85530.48555346452870995No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACTTT63070.3580481352487518No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCCTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG52620.29872352745821024No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC51880.2945225504472054No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG47250.2682380591486209No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46840.2659104908046858No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46310.2629016829454526No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTACTTACTACTGGAGCTGGATCCG45340.25739499686345974No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC44290.2514341511046015No Hit
CTCCTGCACTGGAACCAGCAGTGACTTTGTCTCCTGGTACCAACAATACCCAGGCAAAGCCCCCAAACTCATGA43350.24609777490143314No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTG39570.2246387301695435No Hit
ACCTAGGACGGTCAGCTTGGTCCCTGCGCCGAAAATCACGTTGTTGCGTGTATATAAACTGCAGTAATAATCAGC38410.21805341485499533No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC36320.20618849329688702No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT35770.20306614551843746No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC35590.20204428624549034No Hit
GTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCA35530.2017036664878413No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG35320.20051149733606966No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGTTTATATACACGCAACAACGTGATTTTCGGCG35240.20005733765920425No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACTTTGTCTCCTGGTACCAACAATACCCAGGCAAAGCCCC32870.18660285723206707No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG31740.18018785179634345No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT31200.17712227397750205No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG30920.1755327151084732No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCCTGTCTGGGTCTCCTGGACAGTCGATCAC29860.16951509939000678No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT29820.16928801955157408No Hit
GACTCAGCCTGCCTCCCTGTCTGGGTCTCCTGGACAGTCGATCACCATCT29630.1682093903190188No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28460.16156730504486247No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28290.1606022157315235No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG28090.15946681653936004No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26640.15123517239617484No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTACTTACTACTGGA26210.14879406413302335No Hit
GCTTGGTCCCTGCGCCGAAAATCACGTTGTTGCGTGTATATAAACTGCAGTAATAATCAGCCTCGTCCTCAGC25860.1468071155467373No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25790.14640972582948006No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT25460.14453631716241033No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25190.14300352825298965No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25140.1427196784549488No Hit
GCCCTGACTCAGCCTGCCTCCCTGTCTGGGTCTCCTGGACAGTCGATCAC24720.14033534015140547No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT24600.1396541006361074No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC24500.13908640104002565No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG24110.13687237261530688No Hit
CTTATGATGTGACTAATCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCC23260.1320469260486121No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT23210.13176307625057124No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23070.13096829681605682No Hit
GTTTATATACACGCAACAACGTGATTTTCGGCGCAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCC22420.12727824944152552No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC22370.12699439964348463No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC22260.12636993008779474No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG21760.12353143210738605No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC21540.12228249299600623No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21320.12103355388462642No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT21160.12012523453089562No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21150.12006846457128745No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20990.11916014521755668No Hit
ACAATACCCAGGCAAAGCCCCCAAACTCATGACTTATGATGTGACTAATCGGCCCTCAGGGGTTTCTAATCGCTT20850.11836536578304226No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20380.11569717768145808No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC20330.1154133278834172No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC20120.11422115873164557No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC20030.113710229095172No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATT19960.1133128393779148No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG19530.11087173111476332No Hit
CATCATAAGTCATGAGTTTGGGGGCTTTGCCTGGGTATTGTTGGTACCAG19390.11007695168024888No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT18730.10633013434610943No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC18660.10593274462885219No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18610.10564889483081132No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18540.1052515051135541No Hit
GGAGTGGATTGGGCGTTTTTATACCAGTGAGAGTACCAACTACAACCCCT18530.10519473515394594No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18520.10513796519433777No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC18510.10508119523472959No Hit
CTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCCTGTCTGGGTCTCCTGGA18420.10457026559825602No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC18260.10366194624452525No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC17970.10201561741588822No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCCTGTCTGGGTCTCCTGGACAGTCG17760.10082344826411656No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA17700.10048282850646752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10900.042.0072941
GTGGTAT4200.039.3451771
GTATCAA25200.038.3888741
TGGTATC4450.037.1347732
CCTTTGT12800.037.08722770
TCGTATG1950.035.4688640
CTTATAC2350.033.694541
TATCAAC29400.032.9047472
CGCTAAT2250.032.1903832
TCGCTAA2300.031.47537231
CGTATGC2200.031.44727541
TTATACA2750.031.29732
ATCAACG31750.030.686143
TCAACGC32500.030.0839274
CAACGCA33150.029.4940475
CTGGAGT25200.028.81099370
AACGCAG34300.028.5051776
GTAGTTT2452.9522198E-928.49438770
GTATGTA11850.027.599731
CTCGCTA2800.027.0767130
Produced by FastQC (version 0.11.5)