FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1761495
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTGCGCCGAAAATCAC79490.45126440892537306No Hit
GACCTAGGACGGTCAGCTTGGTCCCTGCGCCGAAAATCACGTTGTTGCGTGTATATAAACTGCAGTAATAATCAG77520.44008072688256283No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCCTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG58360.331309484273302No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACTTT58270.3307985546368284No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC56910.32307784013011676No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56280.3195013326748018No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46540.26420739201644056No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG45530.25847362609601504No Hit
CTCCTGCACTGGAACCAGCAGTGACTTTGTCTCCTGGTACCAACAATACCCAGGCAAAGCCCCCAAACTCATGA43820.24876596300301732No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC42430.24087493861748116No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41740.2369578114045172No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTACTTACTACTGGAGCTGGATCCG40840.23184851503978157No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC39010.22145961243148576No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTG37760.21436336748046403No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT35920.20391769491256007No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG35160.19960317798233887No Hit
ACCTAGGACGGTCAGCTTGGTCCCTGCGCCGAAAATCACGTTGTTGCGTGTATATAAACTGCAGTAATAATCAGC35030.1988651685074326No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCCTGTCTGGGTCTCCTGGACAGTCGATCAC34090.19352879230426426No Hit
GTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCA33810.19193923343523542No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33720.19142830379876183No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG33100.18790856630305508No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGTTTATATACACGCAACAACGTGATTTTCGGCG32980.187227326787757No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACTTTGTCTCCTGGTACCAACAATACCCAGGCAAAGCCCC31450.17854152296770642No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT31080.17644103446220397No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29660.16837970019784332No Hit
GACTCAGCCTGCCTCCCTGTCTGGGTCTCCTGGACAGTCGATCACCATCT29010.16468965282331202No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28610.16241885443898507No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG28090.15946681653936004No Hit
GCCCTGACTCAGCCTGCCTCCCTGTCTGGGTCTCCTGGACAGTCGATCAC26090.1481128246177253No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT26030.14777220486007625No Hit
GCTTGGTCCCTGCGCCGAAAATCACGTTGTTGCGTGTATATAAACTGCAGTAATAATCAGCCTCGTCCTCAGC25970.1474315851024272No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25940.1472612752236027No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTACTTACTACTGGA25750.14618264599104736No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25750.14618264599104736No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG25310.14368476776828773No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25090.1424358286569079No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC24290.137894231888254No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG23980.13613436314040062No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23340.1325010857254775No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23170.13153599641213853No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22920.13011674742193421No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22830.12960581778546063No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC22690.1288110383509462No Hit
CTTATGATGTGACTAATCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCC22320.12671054984544378No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22270.12642670004740292No Hit
GTTTATATACACGCAACAACGTGATTTTCGGCGCAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCC22130.1256319206128885No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC21390.12143094360188363No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC21180.12023877445011197No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCCTGTCTGGGTCTCCTGGACAGTCG20640.1171731966312706No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT20470.11620810731793164No Hit
CTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCCTGTCTGGGTCTCCTGGA20470.11620810731793164No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20330.1154133278834172No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20300.11524301800459268No Hit
ACAATACCCAGGCAAAGCCCCCAAACTCATGACTTATGATGTGACTAATCGGCCCTCAGGGGTTTCTAATCGCTT20240.11490239824694365No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20140.1143346986508619No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19670.11166651054927774No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19540.1109285010743715No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC19240.10922540228612627No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC19010.10791969321513827No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC18980.10774938333631374No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18890.1072384536998402No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC18750.10644367426532576No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC18520.10513796519433777No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA18050.1024697770927536No Hit
GATTATTACTGCAGTTTATATACACGCAACAACGTGATTTTCGGCGCAGGGACCAAGCTGACCGTCCTAGGTCAG17980.10207238737549638No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17930.10178853757745551No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTGCGCCGAAAAT17930.10178853757745551No Hit
ATAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGGAACAGGGAGA17770.10088021822372473No Hit
GGAGTGGATTGGGCGTTTTTATACCAGTGAGAGTACCAACTACAACCCCT17720.10059636842568385No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT17680.10036928858725117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4700.050.5590361
TGGTATC5000.047.4876942
CCTTTGT15300.045.01366870
GGTATCA12900.040.045081
GTATCAA28200.036.5148621
TATCAAC32000.032.045592
ACGAGTC652.3675413E-531.75622722
GACGAGT652.368001E-531.75532521
TCAACGC34400.029.6887384
ATCAACG34550.029.5640373
CAACGCA35350.028.8908775
AACGCAG36700.028.2029256
GTATGTA11500.027.850481
AGTGCGT500.00764232227.66899741
TATGTAC11250.027.5290992
CTGGAGT22850.026.72245470
ACGCAGA38850.026.6421457
CGCAGAG39100.026.5597468
TATGACG658.826468E-426.4515044
CAGAGTA40500.025.72653610
Produced by FastQC (version 0.11.5)