FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218778
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCTGCGGCGGTCACAGACGTCAGCCTCAGGGAGAAGAAGTTCTCGGACGTGTCTACGGA40160.32951037842822894No Hit
GTGTCTGGTGGCTCCATCATCAGCACTAATTACTACTGGGGCTGGATCCG37350.3064544978658952No Hit
GTTTATTACTGTGCGAGACTCCCAGCTCCCCTTTGCAGTGGCCTCAGCTG30550.2506609078929879No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28390.23293823813688794No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT27400.22481534783200877No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25950.2129181852642565No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25030.20536964073851022No Hit
GTATAATAACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT24910.20438504797428242No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22990.18863156374663803No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22660.18592393364501164No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC22340.18329835294040422No Hit
GTCCGTAGACACGTCCGAGAACTTCTTCTCCCTGAGGCTGACGTCTGTGACCGCCGCAGACACGGCTGTTTATTA20720.17000635062332925No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20320.16672437474257001No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA19760.16212960850950706No Hit
CAACTGCACTGTGTCTGGTGGCTCCATCATCAGCACTAATTACTACTGGG18930.15531950855693163No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18420.15113498930896357No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT18130.1487555567954131No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17730.14547358091465387No Hit
GTCTACGGACATGGCGACTCGACTCTTGAGGGACGGGTTGAAGTAGGTGGTCCCACCAAAATTGATACTTCCAAT17270.1416993086517807No Hit
GTTATTATACTGCTGACAGTAATAAAATGCAAAATCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT17020.1396480737263062No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA16860.13833528337400247No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG16600.13620199905150898No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16540.13570970266939508No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA16080.13193543040652195No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15990.1311969858333511No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15790.12955599789297148No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15700.12881755331980066No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14980.12290999673443401No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA14870.12200745336722521No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14780.12126900879405438No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14560.1194639220596368No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14420.11831523050137105No Hit
GTGTTAGCAGCAACTTAGCCTGGTATCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCA14280.1171665389431053No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT14030.11511530401763079No Hit
GTCCCTCAACTGCACTGTGTCTGGTGGCTCCATCATCAGCACTAATTACT13930.11429481004744096No Hit
GTCTCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT13730.11265382210706133No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13600.11158717994581457No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13330.10937184622630208No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13140.10781290768294143No Hit
GTCTGAGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGATT13050.1070744631097706No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC12940.10617191974256182No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA12830.10526937637535302No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGGCCAGTTATTATACTG12810.10510527758131505No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT12780.10485912939025811No Hit
CTGCAAAGGGGAGCTGGGAGTCTCGCACAGTAATAAACAGCCGTGTCTGC12550.10297199325882153No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG12530.10280789446478358No Hit
AATTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGAAGTATCAATTTTGGTGGG12370.10149510411247988No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA12360.1014130547154609No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG12330.10116690652440395No Hit
GGGTGCCACGGCCCCAGAGATCGAAGTACCAGTAGAGGCAGCTGAGGCCACTGCAAAGGGGAGCTGGGAGTCTCG12310.10100280773036599No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC12240.10042846195123312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC24800.035.4543082
ATCAACG26050.034.0181353
GTGGTAT3150.033.8025671
CGACCGG1002.155389E-433.6465170
TCAACGC26750.033.12934
CAACGCA26650.033.124725
AACGCAG26950.032.755996
TAGGCGT550.004610594430.6826969
CGCAGAG29050.030.5063278
AGAGTAC28700.030.2774511
GCAGAGT30000.029.4257939
ACAGTTG1154.891509E-429.25783770
CAGAGTA30050.028.9184210
TCTTAAC854.5849356E-628.2852382
TCGCTAA500.00740412827.848449
GTAGTTT1455.7986734E-527.84538870
TCGTATG756.0429244E-527.6803640
TGTATAA1503.6379788E-1127.4770892
AGTACGG24650.027.30988313
ACGTGTC11900.027.22933463
Produced by FastQC (version 0.11.5)