FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218778
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCTGCGGCGGTCACAGACGTCAGCCTCAGGGAGAAGAAGTTCTCGGACGTGTCTACGGA40200.32983857601630484No Hit
GTGTCTGGTGGCTCCATCATCAGCACTAATTACTACTGGGGCTGGATCCG37490.3076031894241609No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30500.250250660907893No Hit
GTTTATTACTGTGCGAGACTCCCAGCTCCCCTTTGCAGTGGCCTCAGCTG29510.24212777060301383No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29340.24073293085369116No Hit
GTATAATAACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT25510.2093080117954213No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23220.1905186998780746No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC22850.18748287218837228No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22440.18411884691059408No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20250.16615002896343714No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20190.16565773258132324No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA19730.16188346031845013No Hit
GTCCGTAGACACGTCCGAGAACTTCTTCTCCCTGAGGCTGACGTCTGTGACCGCCGCAGACACGGCTGTTTATTA19520.16016042298105151No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18480.15162728569107745No Hit
GTTATTATACTGCTGACAGTAATAAAATGCAAAATCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT18460.1514631868970395No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17910.14695047006099551No Hit
CAACTGCACTGTGTCTGGTGGCTCCATCATCAGCACTAATTACTACTGGG17550.1439966917683122No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16830.13808913518294552No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16320.1339046159349775No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16180.13275592437671174No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16120.13226362799459787No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT16080.13193543040652195No Hit
GTCTACGGACATGGCGACTCGACTCTTGAGGGACGGGTTGAAGTAGGTGGTCCCACCAAAATTGATACTTCCAAT15860.13013034367210435No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15510.12725861477644002No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15400.12635607140923122No Hit
GTGTTAGCAGCAACTTAGCCTGGTATCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCA15260.12520737985096547No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG15220.12487918226288955No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15040.12340229311654789No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14980.12290999673443401No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA14970.122827947337415No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14840.12176130517616826No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT14710.12069466301492152No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT14580.11962802085367474No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14560.1194639220596368No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA14340.11765883532521919No Hit
GTCTGAGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGATT14300.11733063773714328No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA14240.1168383413550294No Hit
GTCCCTCAACTGCACTGTGTCTGGTGGCTCCATCATCAGCACTAATTACT14110.11577169919378262No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14000.11486915582657384No Hit
GGGTGCCACGGCCCCAGAGATCGAAGTACCAGTAGAGGCAGCTGAGGCCACTGCAAAGGGGAGCTGGGAGTCTCG13740.11273587150408032No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT13670.11216152572494743No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC13140.10781290768294143No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13100.10748471009486552No Hit
GTCTCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT13040.10699241371275163No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGGCCAGTTATTATACTG12850.10543347516939099No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA12840.105351425772372No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA12780.10485912939025811No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12770.10477707999323914No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12700.10420273421410625No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT12570.10313609205285951No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG12550.10297199325882153No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA12440.10206944989161275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3450.039.8478281
TATCAAC24400.037.5775572
ATCAACG25200.036.0972563
TCAACGC25200.035.9595684
CAACGCA26100.034.7195785
AACGCAG26500.034.5840956
CGCAGAG27900.032.848698
AGAGTAC28250.032.80488611
GCAGAGT29100.031.7300199
TGCGTAC450.004211536631.25619562
GTACGCT553.3557025E-431.204584
CAGAGTA29950.030.94410910
GTAGTTT2001.5053956E-830.92552270
TGTTAGA4900.030.85539870
ACGCAGA30850.029.5963067
TTCGGGG13050.027.91064670
CTGGAGT22550.027.42840270
AGTACGG26250.027.32814813
GAGTACG27050.026.773712
CTACGGA14550.026.29081269
Produced by FastQC (version 0.11.5)