FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849220
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12650.14896022232165987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24800.035.655991
GGTATCA12600.031.8258361
ATCAACG28450.030.5689813
TTAGGCG450.00473872330.51071730
TCAACGC28550.030.458324
CAACGCA29000.030.1037485
TATCAAC29300.030.0380522
AACGCAG29550.029.6592986
ACGCAGA31150.028.1358667
AGAGTAC31400.027.58476311
CAGAGTA31800.027.23778510
CGCAGAG32550.026.9257228
GTGGTAT5700.026.4571041
GCAGAGT34250.025.689229
TAGTATA951.1123684E-525.2336852
TGGTATC6100.024.7018282
CCGCTAT1651.4889339E-424.23650670
GAGTACG28150.023.83724412
AGTACGG28600.023.46218113
TACGGGG14300.022.0270215
Produced by FastQC (version 0.11.5)