FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10720
Sequences flagged as poor quality0
Sequence length20-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAACCC150.002357952868.50236535
CGCAGAG856.548362E-1139.730628
GGTATCA350.001431609638.595461
ACGCAGA901.200533E-1037.5233657
GTATCAA901.200533E-1037.5233651
TATCAAC901.200533E-1037.5233652
TCAACGC952.1645974E-1035.548454
AGAGTAC952.1645974E-1035.5484511
CAACGCA952.1645974E-1035.548455
AACGCAG1003.7471182E-1033.7710276
CAGAGTA1003.7471182E-1033.77102710
GCAGAGT1003.7471182E-1033.7710279
ATCAACG1003.7471182E-1033.7710273
AGTACGG1151.7481962E-623.4928913
GAGTACG1151.7481962E-623.4928912
GTACGGG1202.531664E-622.51401914
Produced by FastQC (version 0.11.5)