FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2142724
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41120.19190525704663783No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30290.14136211663284678No Hit
GTCTGGGACAGACTTCGCTCTCACCATCAGCAGACTGGAGCCTGAAGATT26930.12568114232164293No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23710.11065354194007254No Hit
GTATCACTGTCAGCAGTATGGTAACTCACCTGAAACTTTTGGCCAGGGGA23570.11000016801043905No Hit
GTATGGTAACTCACCTGAAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT23540.10986015931123186No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23450.10944013321361035No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23130.10794670708873379No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22280.10397979394453041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)