FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457478
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9820.21465513095711708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12700.040.40411
TATCAAC14600.035.12682
CCATACG400.00272228934.1639143
TACGTGT400.00272813334.148988
ATCAACG15100.033.711413
AACGCAG15250.033.5891576
TCAACGC15300.033.2561954
CAACGCA15450.033.1543465
AGAGTAC15950.032.32912411
ACGCAGA16150.031.9287667
CGCAGAG16200.031.6194258
CAGAGTA16650.030.96994610
CATACGG450.00485677430.3546474
GCAGAGT17650.029.0217939
CTAGCAC605.736485E-428.469933
ACTAGCG605.751183E-428.45748313
CTTATAG1000.008847753526.8409870
GAGTACG14750.026.39310812
AGTACGG14850.026.21537813
GGTATCA6550.026.113591
Produced by FastQC (version 0.11.5)