FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799854
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAGCTGCTCATTTACTGGGCTTCTACCCGGGAATTCGGGGTCCCT20080.25104581586139474No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCATTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17670.2209153170453608No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13820.17278153262970491No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGGAGTTCTTATTGTTGGAGCTGTATAAAATACT13760.17203139572972068No Hit
ATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA13230.1654051864465265No Hit
ATTTTATACAGCTCCAACAATAAGAACTCCTTAGCTTGGTACCAGCAGAA12510.15640354364671552No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12330.1541531329467628No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC12280.15352801886344258No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCATTGTACTTTGGCCT11560.1445263760636316No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10920.136524915797133No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10790.13489961918050045No Hit
ATATTATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT10490.1311489346805792No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG10260.12827340989730626No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9960.12452272539738501No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9510.11889669864750317No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9500.11877167583083913No Hit
GAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT9220.11527103696424598No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8450.1056442800811148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTAG551.5196565E-743.7201421
GTATCAA15300.040.4135781
GGTATCA6750.037.150561
TATCAAC16550.036.9114072
ATCAACG18500.032.631353
TCAACGC18350.032.5201844
TTTCGAT850.00399804431.58528370
AACGCAG19650.031.0668546
CAACGCA19300.030.9194495
ACGCAGA20900.029.0446937
CGAACTA605.5857847E-428.60300624
CAGTTCG8800.028.22037570
AGAGTAC21550.028.16863611
GTGGTAT3100.027.7028581
CGCAGAG21950.027.4990678
ACCCTAC756.344815E-527.4812361
TGGTATC3200.026.8119622
GCAGAGT23000.026.2436759
AGTACGG19450.024.50953313
GAGTACG19850.024.01563612
Produced by FastQC (version 0.11.5)