FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2670002
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGTTGGCAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC37010.13861412837893006No Hit
GTATAATAGTTACCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT32620.12217219312944336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85400.040.41861
GGTATCA34450.039.4196971
TATCAAC93800.036.7990232
GTGGTAT11550.036.612431
ATCAACG97750.035.2768333
TCAACGC98800.034.9025764
CAACGCA100450.034.3292665
AACGCAG102900.033.612776
TGGTATC13500.030.5599672
ACTACGC3050.030.45354527
ACGCAGA114350.030.213627
CGCAGAG116500.029.6265228
CTACGCT3150.029.49284628
AGAGTAC116050.029.4440711
CAGAGTA120050.028.5494510
GCAGAGT123400.027.969419
AGTACGG78050.026.02933513
GAGTACG78700.025.68379612
TCAAACG11800.025.07804548
TACGCTG4450.023.2009729
Produced by FastQC (version 0.11.5)