FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1730182
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67950.042.0551761
GTGGTAT9500.038.953191
TATCAAC76800.037.2089732
GGTATCA28200.037.058991
TCAACGC80800.035.1559334
ATCAACG81050.035.131033
CAACGCA82200.034.5581635
AACGCAG84600.033.4157756
TCGTATG1101.024091E-931.46499340
ACGCAGA91750.030.8090427
AGAGTAC91000.030.61025211
TATACCG450.004779580530.4589845
CGCAGAG93500.030.2690478
CAGAGTA92200.030.21272710
GCAGAGT96300.029.3889489
AGTACGG66500.028.13041913
TGGTATC13250.027.92872
GAGTACG67050.027.84857212
TAAGGCG2850.027.70480229
CTCGTAT1251.2389319E-724.89411239
Produced by FastQC (version 0.11.5)