FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1730182
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29840.17246740516315626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9700.042.0340041
GGTATCA32700.039.1876641
GTATCAA67900.037.744821
TATCAAC76650.033.3225362
TGGTATC12850.031.440562
CAACGCA82350.031.0758445
ATCAACG82250.031.0747243
TCAACGC82350.031.0342984
AACGCAG85650.029.998366
ACGCAGA92300.027.7629177
CGCAGAG93850.027.3408458
CAGAGTA92750.027.18557410
AGAGTAC93150.027.03210611
GCAGAGT97350.026.3578669
TATACCG700.001400771224.4375085
AGTACGG70000.024.14425713
GAGTACG71450.023.60639412
CAAACGA3200.021.84686749
GTACGGG76050.021.59369314
AATAGCG3050.020.8857162
Produced by FastQC (version 0.11.5)