FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575794
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30130.19120519560297855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11150.044.472411
GTATCAA79900.040.0186421
TATCAAC86650.036.518152
GGTATCA33400.036.450291
TGGTATC13500.036.4497952
ATCAACG93600.033.858113
TCAACGC93750.033.795354
CAACGCA95150.033.2632755
AACGCAG97500.032.4965636
ACGCAGA104650.030.27637
CGCAGAG105750.029.960428
AGAGTAC106350.029.5666711
CAGAGTA107350.029.32305110
GCAGAGT108750.029.165329
ACGTTAG950.007160549528.03880370
AGTACGG71450.026.85433413
GAGTACG71950.026.81006812
CCTATAC2800.024.3928473
GTACGGG78450.023.93591514
TACGGGG26950.023.5632115
Produced by FastQC (version 0.11.5)