FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943655
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18360.1945626314701877No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16850.17856102071201868No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13670.14486226428090776No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12680.13437114199575056No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA12620.13373531640271075No Hit
GTCTGGGACAGACTTCGCTCTCACCATCAGCAGACTGGAGCCTGAAGATT12410.13150992682707133No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10540.11169336251066332No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT10480.11105753691762349No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGATTACTACTGGAGCTGGGTCCG10240.10851423454546417No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10160.10766646708807773No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAATAAAGGTGAGCCACCATAGTG10040.10639481590199809No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9480.100460443700293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16900.039.6228871
GGTATCA7100.035.7908131
TATCAAC19750.033.183252
ATCAACG20250.032.6889343
TCAACGC20450.032.5335044
CAACGCA20950.031.7553675
AACGCAG21300.031.5555616
TAGATCG450.004759451430.4838589
AGAGTAC23450.028.66240711
CGCAGAG24000.028.0070448
TCGGTAT500.007965242527.4340214
GCAGAGT24900.027.1324719
CAGAGTA24900.026.99474310
ACGCAGA25050.026.8316757
TTCGATT550.00979331326.2957767
GTGGTAT4250.025.8558771
GAGTACG21200.025.39587812
GCACTAT8500.025.31849970
AGTACGG21400.025.15853313
AAATCGA850.0012353624.96801669
Produced by FastQC (version 0.11.5)