FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2616290
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA111850.041.072751
GTGGTAT14200.040.5002941
GGTATCA47200.039.0191231
TATCAAC126650.036.300122
TCAACGC132400.034.673264
ATCAACG133800.034.4114843
CAACGCA134750.034.1213345
TCGTATG5450.033.6542840
AACGCAG139850.032.901496
CTCGTAT5200.031.91387439
TATACAC8800.031.5093613
ACGCAGA151200.030.4299567
TGGTATC18800.030.2264752
CGCAGAG153200.030.0550448
AGAGTAC152700.029.99537811
CAGAGTA153050.029.95029310
CTTATAC8800.029.1753351
GCAGAGT159100.028.9620139
CGTATGC6350.027.8320541
ACGGACT8550.027.656827
Produced by FastQC (version 0.11.5)