FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880946
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTCTTGTTGGTACTGTATAAAACATT46060.5228470303514631No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGCAATCCGGGGTCCCT42420.4815278121473961No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40570.46052766003818624No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36480.4141002967264736No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33630.38174871104471786No Hit
GTACCAACAAGAGGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGG31560.35825124354954785No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31060.35257552676327497No Hit
GTTTTATACAGTACCAACAAGAGGAACTACTTAGCTTGGTACCAGCAGAA29240.3319159176612414No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26600.30194813302972034No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26520.30104001834391664No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24670.2800398662347068No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24580.27901823721317764No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24430.27731552217729577No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22470.25506671237510586No Hit
GCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC21790.24734773754577466No Hit
GTATAAAACATTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAATCTGGAGA20470.23236384523001413No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20410.23168275921566134No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20380.23134221620848497No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20160.22884490082252487No Hit
ATATTATAGTACTCCTCTGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT20080.2279367861367212No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC19870.22555298508648655No Hit
CCTCTGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG19550.2219205263432719No Hit
GTCAGGGACCCCGGATTGCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA19140.2172664385785281No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18970.21533669487119525No Hit
CACCATCACCGGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATATTATAGTACTCCTCTGACGTT18810.21352046549958795No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTGGGCCGAACGTC18760.21295289382096066No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17580.19955820220535653No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC17550.19921765919818013No Hit
TTATAGTACTCCTCTGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT17310.19649331514076915No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17130.19445005709771088No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16550.18786622562563426No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16340.18548242457539962No Hit
CTATAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCCGGTGATGGTGAGAGTGAAATCTGTC16290.1849148528967723No Hit
TGTTGGTACTGTATAAAACATTCTGGCTGGACTTGCAGTTGATGGTGGCC16130.18309862352516498No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGCAA15830.17969319345340123No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15490.17583370603873563No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA15080.17117961827399183No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCACCGGCC14950.16970393190956087No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT14630.16607147316634618No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTC14330.1626660430945824No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14210.1613038710658769No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTCTTGTTGGTACTG14120.1602822420443478No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14120.1602822420443478No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13950.15835249833701498No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13280.15074703784340923No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12760.14484429238568539No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12630.14336860602125442No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12630.14336860602125442No Hit
TCTTCAGCCTGCAGGCCGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC12420.1409848049710198No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12270.1392820899351379No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12240.13894154692796154No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12110.13746586056353058No Hit
GCTCATTTACTGGGCATCTACCCGGCAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT12060.13689828888490327No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11940.13553611685619776No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTATACAGTACCAACAAGAGGAACTA11650.13224420112015947No Hit
CCACTGAATCGGTCAGGGACCCCGGATTGCCGGGTAGATGCCCAGTAAAT11650.13224420112015947No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11500.1305414860842776No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT11350.1288387710483957No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11220.12736308468396473No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11130.1263414556624356No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATT11130.1263414556624356No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10930.12407116894792643No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10890.12361711160502459No Hit
CTTCGGGGACATCGTGATGACCCAGTCTCCAGATTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG10620.12055222454043721No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10580.12009816719753537No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10540.11964410985463356No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT10490.11907653817600625No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA10440.11850896649737896No Hit
GTACTATAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCCGGTGATGGTGAGAGTGAAAT10380.1178278804830262No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10350.11748733747584982No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10340.11737382314012436No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10290.11680625146149708No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10220.11601165111141887No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10150.11521705076134064No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10140.11510353642561519No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9970.11317379271828239No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG9910.11249270670392963No Hit
CGTCAGAGGAGTACTATAATATTGCTGACAATAATAAACTGCCACATCTT9900.11237919236820418No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9890.11226567803247874No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9790.11113053467522414No Hit
GATTTCACTCTCACCATCACCGGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATATTATAGTACT9620.10920079096789134No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9510.1079521332749113No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9410.1068169899176567No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTGCCGGGT9410.1068169899176567No Hit
GGATCTCTGGTGCCTTCGGGGACATCGTGATGACCCAGTCTCCAGATTCCCTGGCTGTGTCTCTGGGCGAGAGGG9370.10636293257475488No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9300.10556833222467665No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9300.10556833222467665No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9190.1043196745316966No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTTCGGGGACATCGTGATGA9150.10386561718879476No Hit
GTCCCTGGGCCGAACGTCAGAGGAGTACTATAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCTGCAGG9060.10284398816726564No Hit
GGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATATTATAGTACTCCTC8850.100460187117031No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG350.00474138957.82610370
CTCGTAT707.2759576E-1249.36926339
CGTATGC900.046.16520341
TCGTATG950.043.69535440
GTAGTTT1259.798896E-943.17682370
ACTAGAT803.6379788E-1142.96441732
GGTATCA7250.041.5920371
CGTACTA1000.041.19423329
ACCGTAC1000.041.16847627
CGAAATT702.0243533E-839.77781348
GACCGTA955.456968E-1239.71268526
TGGTATC2350.039.3696372
TACTAGA1100.037.4770131
CAACCGA753.8082362E-837.0256144
GTACTAG1151.8189894E-1235.83737630
GTGGTAT2500.035.636811
GTATCAA15200.035.393341
CCGAAAT806.60184E-834.7815247
TAATCGT709.712676E-734.5090938
TCACGTA701.021439E-634.28757525
Produced by FastQC (version 0.11.5)