FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405778
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49940.20758357587441567No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT47890.19906242388117276No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA43950.1826851854161107No Hit
GTGTATTACTGTGCCAGAACCTACGGTGACTACGGCATCCTGAATGATGC42410.17628392977240626No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT39830.16555974823944689No Hit
GTAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTCCAGATATG38150.15857656026449657No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37080.15412893458997462No Hit
TTATTATACTGCTGACAGTAATAAACCGCAAAATCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC36210.15051264081723253No Hit
GTATAATAATTGGCCTCCGGTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT36120.1501385414614316No Hit
GTCCCAGACCCACTGCCAGTGAACCTGGCTGGGATACCAGTGGCCCTGGT34370.14286438732085835No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT33090.13754386314946765No Hit
GTCTCCAGCCATCCTGTCTGTGTCTCCAGGGGAAGGAGCCACCCTCTCCT32710.13596433253608603No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGATT32430.13480046787359432No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31530.13105947431558523No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31250.12989560965309352No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30730.12773414670846606No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGACCGGAGGCCAATTATTATACTG30630.12731848075757612No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30190.12548955057366057No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGAAAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT29710.12349435400938907No Hit
GATCAGCACTGAGCACCGAGGACTCACCATGGAACTGGGGCTCCGCTGGG29480.12253832232234228No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC28040.11655273262952774No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27690.11509790180141309No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGATTTTGCGGTTTATTACTGTCAGCAGTATAATAATTGG26760.11123220845813703No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26130.10861351296753068No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC26040.10823941361172976No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG25970.10794844744610682No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG25700.1068261493787041No Hit
CTGGTATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGAGTTCACT24390.10138092542204642No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)