FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592161
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9710.16397567553418751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3400.044.4585951
GTATCAA24700.039.3614851
GGTATCA10600.038.8917351
TGGTATC3800.038.8418242
TATCAAC28500.033.9640122
ATCAACG29550.032.8427933
TCAACGC29750.032.5039834
CAACGCA30200.032.0223585
CCGTTTA450.00433231531.07346552
AACGCAG31100.030.98546
TCCGTAA450.004731183430.51914625
ACGCAGA32750.029.4243017
CGCAGAG32900.029.3943828
AGAGTAC33200.029.02547611
CAGAGTA33700.028.39131210
GCAGAGT34550.028.2883729
TCCCGTA756.375129E-527.4602642
GAGTACG24650.026.43085112
AGTACGG25200.025.85398513
GTATACC809.832833E-525.765781
Produced by FastQC (version 0.11.5)