FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362482
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15500.041.403231
GTATCAA34850.037.6170651
TCAACGC40250.032.826094
ATCAACG40600.032.6276363
CAACGCA41450.031.875765
TATCAAC41950.031.4958342
AACGCAG43200.030.6639376
ACGAACC1900.029.4431152
ACGCAGA46650.028.3920147
CGCAGAG47800.027.5653748
AGAGTAC47200.027.1161111
TAATCGT2200.026.72359738
GTATGCG658.929191E-426.3986051
CAGAGTA49700.025.75212110
GCAGAGT51100.025.7180759
GAGTACT21850.022.45666112
GTGGTAT6200.021.5872461
GAGTACG36700.021.50415412
AGTACTT22650.021.36050413
AATCGTT2600.021.30262839
Produced by FastQC (version 0.11.5)