FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362482
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18880.13857063799741942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40550.037.748621
ATCAACG44500.034.126393
TCAACGC44950.033.8523144
TATCAAC45200.033.8428042
CAACGCA45800.033.2976575
AACGCAG46450.032.8317036
ACGCAGA49950.030.6684027
GTGGTAT8200.030.5538621
AGAGTAC50400.029.91969711
GGTATCA17600.029.8356761
CAGAGTA51550.029.25223510
CGCAGAG52500.029.0493078
GCAGAGT54550.028.0204529
GAGTACG42700.025.84419812
AGTACGG43600.025.7037413
ACGGGTA4700.024.79589816
TGGTATC10200.023.5379032
TAGCGCT750.002072111722.846937
GTACGGG50950.021.65941414
GCCGTTA1455.6396675E-721.27362315
Produced by FastQC (version 0.11.5)