FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580806
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40350.037.107681
GGTATCA19350.032.5808751
TATCAAC46500.032.051512
TCAACGC47800.031.1091214
ATCAACG47900.031.0431923
CAACGCA49200.030.2239025
AACGCAG50800.029.3394156
ACGCAGA55150.027.0218247
AGAGTAC55850.026.25293411
CGCAGAG57200.026.0533878
CAGAGTA56100.026.01463310
GCAGAGT62050.024.0169819
TTAGCGC902.228462E-422.84202821
GAGTACG46000.021.74614312
AGTACGG45850.021.74256913
ACGGGTA2058.185452E-1121.72434616
GTATTAG4350.021.2667161
TAACGGC2151.4006218E-1020.85706536
CGAATTA1004.5470128E-420.55457315
GCGTAGA2359.5874384E-519.08781470
Produced by FastQC (version 0.11.5)