FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580806
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22830.1444199984058765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46800.036.306841
TATCAAC53850.031.6001782
ATCAACG54550.031.1798743
TCAACGC54700.031.1480644
GGTATCA22450.030.97651
CAACGCA55650.030.6163375
AACGCAG56250.030.1681186
GTCGATT1104.1310317E-430.11573870
ACGCAGA59400.028.5682937
CGCAGAG59400.028.4530968
AGAGTAC59450.027.62348211
CAGAGTA60750.027.14499710
GTGGTAT11500.026.214251
GCAGAGT65000.025.949129
TGGTATC12300.024.7744352
TATACTG3050.024.6781125
AGTACGG50500.022.83113913
GAGTACG50650.022.69597612
GAGTACT23850.020.65680712
AGTACTT23650.020.54216813
Produced by FastQC (version 0.11.5)