FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867929
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11770.13561017087803265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23800.038.5946771
TATCAAC27450.033.076752
TCAACGC28050.032.7149284
AACGCAG28550.032.5017856
ATCAACG28450.032.1420173
CAACGCA28850.031.9264415
GGTATCA10250.030.7633461
ACGCAGA31200.029.6314727
CGCAGAG31550.029.0856958
CAGAGTA31500.028.6970610
AGAGTAC31600.028.4978911
GTGGTAT4850.026.8541551
GCAGAGT34600.026.8186689
CGAATTA907.3200536E-626.63174215
TGTACCG907.3200536E-626.6317425
AGTACGG24800.024.9902713
GAGTACG25550.024.256712
ACGGGTA1902.910383E-1123.42792116
TACGGGG13200.022.04900715
CGAGTTT1550.003159986821.24157570
Produced by FastQC (version 0.11.5)