FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408541
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12930.3164921023838489No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCA200.006237575353.9526265
TAGTCCG350.001430425838.948289
GGTATCA6600.038.271521
GTATCAA13650.036.2596171
GTTAGCC400.002750112734.0922623
TATCAAC15450.032.003812
ATCAACG15850.030.7583183
TCAACGC16000.030.458774
CAACGCA16400.029.923685
AACGCAG16600.029.563156
ACGCAGA17650.027.6113517
CGCAGAG17750.027.0717968
AGAGTAC17950.026.20058311
GCAGAGT18600.025.8346449
GTGTAAG1209.413452E-825.6005421
CAGAGTA18800.025.19725610
ACGGGCT1251.4172656E-724.54041716
TATAGGG902.297267E-422.733742
TACGGGC2700.022.71982815
TACACCC750.002137859622.7198285
Produced by FastQC (version 0.11.5)