FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906261
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25650.039.260061
TATCAAC28950.035.0214542
TCGTATG806.937262E-834.58635740
CTAATCG400.002620945734.43288434
GTGGTAT4000.033.396091
ATCAACG30650.033.1907423
TCAACGC30750.033.0828064
CAACGCA31500.032.29695
AACGCAG31500.032.1881566
GGTATCA11600.031.5948871
CTCGTAT901.9606887E-730.72630939
CGCGTGA450.004787924230.44657910
ACGCAGA34950.029.107197
AGAGTAC35550.028.4232311
CGCAGAG35950.028.3928138
CAGAGTA36300.027.83597410
GCTAATC1750.027.53103833
GCAGAGT39000.026.2601749
TAGTACG1101.2269629E-624.9108354
GAGTACG28150.023.7259312
Produced by FastQC (version 0.11.5)