FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220164
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5570.2529932232335895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAG200.005219692456.4274468
ACGCGAG200.00644334753.50325465
GCGTAGT200.006558728453.26378363
TATTGGG200.00756516451.3757672
CGAGTTG200.007578769251.35243214
ATACCGT200.007578769251.3524326
GTATCAA7500.042.0330621
ATATTAG350.001391199739.1612361
GCCTAGA451.0397567E-438.0734251
ATCAACG8400.037.4954223
GGTTAAA700.00180445237.12721670
TCAACGC8600.037.0215234
CAACGCA8700.036.202485
TATCAAC8850.035.605052
GGTATCA3000.035.4082831
AACGCAG8950.035.191246
ACGCAGA9100.034.611167
GTATAGG400.002679710734.2660871
CGCAGAG9500.033.153858
GCAGAGT9900.032.1601079
Produced by FastQC (version 0.11.5)