FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2040406
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30910.1514894584705201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48950.037.380651
GGTATCA21700.033.8552741
TATCAAC56600.032.3013652
TCAACGC57100.031.8203584
ATCAACG57700.031.5519753
CAACGCA57850.031.467085
AACGCAG59100.030.91836
ACGCAGA63550.028.8611837
AGAGTAC63550.028.32172211
TGGTATC10250.028.1103042
GTGGTAT11000.028.0880831
CAGAGTA65350.027.6465510
CGCAGAG66600.027.5394638
GCAGAGT70150.026.1458059
AGTACGG51550.022.47777413
AGTACTT28550.022.45436513
GAGTACG52050.022.3282612
GAGTACT29000.021.87004312
CGAATTA2252.8012437E-1019.80830815
GTACTTT32100.019.757514
Produced by FastQC (version 0.11.5)