FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2411541
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT93100.3860601996814485No Hit
GATCTATACTGCATTCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAAATCTGGGACAGATTTCA74830.31029951387929955No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG60900.2525356193404964No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG57210.23723420004055495No Hit
GAGTTACAGTACCCCTCGAACTTTCGGCCCTGGGACAAAAGTCGATATCACACGAACTGTGGCTGCACCAT56540.23445589355519975No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56310.23350214655276438No Hit
GTGCAGCCACAGTTCGTGTGATATCGACTTTTGTCCCAGGGCCGAAAGTT56100.23263133407227993No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC54650.22661858123083956No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT51710.21442720650405694No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT51400.21314172141381799No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC50510.2094511351870028No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG49200.20401892399921875No Hit
GTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAACT47510.19701095689436754No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG47500.1969694896333921No Hit
ATACTGCATTCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAAATCTGGGACAGATTTCACTCTCA46670.19352770697242966No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46500.19282276353584699No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGCCAA45080.18693441247733297No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA44780.1856903946480694No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT44730.18548305834319218No Hit
GCCACAGTTCGTGTGATATCGACTTTTGTCCCAGGGCCGAAAGTTCGAGG44160.18311942446759147No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT43990.18241448103100882No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43450.18017524893833445No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC42530.17636026092859297No Hit
AGTCAGAGCATTGCCAACTATTTAAGTTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATA40800.16918642477983994No Hit
GTTACAGTACCCCTCGAACTTTCGGCCCTGGGACAAAAGTCGATATCACA40640.16852294860423273No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT39670.16450062428961398No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT38370.15910988036280535No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC35150.14575742232871017No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGCCAACTATTTAAGTTGGTATCAGCAGAAA34660.14372552654091306No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34160.1416521634921405No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATATCGACTTTTGTCCCAGGGCCGAAAGTTCGAGGGGTA33320.1381689135702026No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT33290.13804451178727625No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT32890.13638582134825822No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATTTACTGCCA32090.13306844047022215No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC31940.13244643155559038No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA31830.13199029168486043No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC31030.12867291080682436No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30860.1279679673702417No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28910.11988185148002874No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT28790.11938424434832333No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA28270.11722794677759987No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCTCGAACTTTCGGCCCTGGGACAAAAGTCGATATCACACGAA27770.11515458372882734No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACC27480.11395203316053927No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC27360.11345442602883385No Hit
CTATTTAAGTTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGA26850.11133959571908583No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26750.11092492310933134No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG26430.10959797075811689No Hit
ATGCAGTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAACTTAAATAGTTGGCAATGCT26370.10934916719226419No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26310.10910036362641148No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT26220.10872715827763242No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26050.10802221484104976No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA25950.10760754223129525No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCCTCGAACTTT25220.10458043218008733No Hit
GTTTACAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAAATCTGGGACAGATTTCACTCTCACCATCAGCAGT25080.10399989052643102No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24320.10084837869229675No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA24300.10076544417034584No Hit
GAGTGAAATCTGTCCCAGATTTACTGCCACTGAACCTTGATGGGACCCCA24250.10055810786546861No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT24150.10014343525571409No Hit
GTAATACACGGCCGTGTCCTCAGATCCCAGGCTGCTCAGCTCCATGTAGGCTGTACTTATGGAGGTGTTCCAGGT24130.10006050073376319No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)