FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2411541
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT91930.3812085301473207No Hit
GATCTATACTGCATTCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAAATCTGGGACAGATTTCA77510.32141273982072044No Hit
GAGTTACAGTACCCCTCGAACTTTCGGCCCTGGGACAAAAGTCGATATCACACGAACTGTGGCTGCACCAT55820.23147025076496727No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT55070.2283602061918085No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG54820.2273235246674222No Hit
GTGCAGCCACAGTTCGTGTGATATCGACTTTTGTCCCAGGGCCGAAAGTT54010.22396467652841065No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG52550.21791045642599485No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC50910.21110982562602087No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC50680.2101560786235855No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT49700.20609228704799132No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC48070.19933312350899282No Hit
GTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAACT47820.19829644198460653No Hit
ATACTGCATTCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAAATCTGGGACAGATTTCACTCTCA46530.19294716531877334No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA45170.18730761782611202No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44910.1862294690407503No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGCCAA44010.1824974155529597No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGCCAACTATTTAAGTTGGTATCAGCAGAAA43070.17859949302126732No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG42590.17660906449444566No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT42300.1754065139261576No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC42200.17499184131640308No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40740.16893762121398723No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT40470.16781800516765005No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT39790.1649982314213194No Hit
AGTCAGAGCATTGCCAACTATTTAAGTTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATA39440.1635468772871786No Hit
GCCACAGTTCGTGTGATATCGACTTTTGTCCCAGGGCCGAAAGTTCGAGG38780.16081003806279884No Hit
GTTACAGTACCCCTCGAACTTTCGGCCCTGGGACAAAAGTCGATATCACA36910.15305566026038953No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC36560.15160430612624873No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35400.1467941038530964No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33820.14024227661897518No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33180.13758837191654633No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32030.13281963690436946No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATTTACTGCCA31700.13145121729217957No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT31120.1290461161556034No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA30580.12680688406292906No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30510.1265166132361009No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATATCGACTTTTGTCCCAGGGCCGAAAGTTCGAGGGGTA30250.12543846445073917No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT29460.12216255083367855No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA29210.12112586930929227No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC28700.11901103899954427No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC28670.11888663721661792No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT28230.11706207773369809No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA28120.1166059378629681No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC27110.11241774450444758No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27090.11233480998249668No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26970.11183720285079127No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACC26960.11179573558981581No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCTCGAACTTTCGGCCCTGGGACAAAAGTCGATATCACACGAA26570.11017851241177322No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26410.109515036236166No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26390.10943210171421511No Hit
CTATTTAAGTTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGA26120.10831248566787793No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT25810.10702700057763895No Hit
GTTTACAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAAATCTGGGACAGATTTCACTCTCACCATCAGCAGT25560.10599031905325267No Hit
GTAATACACGGCCGTGTCCTCAGATCCCAGGCTGCTCAGCTCCATGTAGGCTGTACTTATGGAGGTGTTCCAGGT25540.10590738453130177No Hit
ATGCAGTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAACTTAAATAGTTGGCAATGCT25460.10557564644349818No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCCTCGAACTTT25120.10416575957033283No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA25040.10383402148252921No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24520.10167772391180578No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)