FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909253
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22050.041.5804861
TATCAAC26200.034.9942672
ATCAACG26700.034.210333
GGTATCA8500.033.5309681
TCAACGC27300.033.4584584
CAACGCA27900.032.8619965
AACGCAG28100.032.507696
CTTATAC2050.030.1512661
ACGCAGA30700.029.9766647
AGAGTAC30700.029.41739811
CGCAGAG32600.028.1242288
GCAGAGT32900.027.76349
GTGGTAT4500.026.7080651
CAGAGTA35150.025.88853510
AGTACGG26400.024.0619513
GAGTACG26550.023.66865212
TATACAC2700.022.8926283
GTGTAAG1704.6820787E-922.2193151
TTATACA3100.022.1541582
CCCTAAG2600.021.1479428
Produced by FastQC (version 0.11.5)