FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514375
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATAC200.00751749651.467823
TCGTATG751.6370905E-1146.1077840
CTCGTAT803.45608E-1143.20910639
GTATCAA11700.038.7146071
CGTATGC852.8685463E-936.65091341
TATACAC1350.035.582693
TACGTCG501.9207194E-434.3118825
TCAACGC14100.032.6084564
ATCAACG14150.032.493233
CAACGCA14450.031.8186325
AACGCAG14650.031.6184566
TTATACA1208.185452E-1131.4525592
TCTCGTA1001.43299985E-831.08011638
TATCAAC14850.030.9615612
GTGGTAT2450.030.8136631
ACGCAGA15650.029.5981087
GGTATCA5250.029.4130421
CAGAGTA15900.029.34852610
AGAGTAC16100.029.19706711
CTTATAC1302.0918378E-1029.0359521
Produced by FastQC (version 0.11.5)