FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514375
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8690.16894289185905223No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTACG200.00755561851.4020116
GGTATCA4950.041.593591
GTATCAA11400.039.130811
TATCAAC13700.032.7799642
ATCAACG13750.032.4019933
CAACGCA13900.031.8026835
TCAACGC13950.031.6886964
AACGCAG14250.031.5025186
ATACGGT450.00475496430.48712527
ACGCAGA14850.029.998937
AGAGTAC15500.028.51982511
CAGAGTA15550.028.20774810
AATAGCG905.631564E-627.54177362
CGCAGAG16200.027.499028
GCCTAAC500.00793683927.4517671
TACGGTT500.00794438227.44642328
TTTCGGG500.00797839727.42240122
AACGAAC959.5970045E-625.71506551
CAACTTA801.0000025E-425.70100614
GCAGAGT17750.025.2907569
Produced by FastQC (version 0.11.5)